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Quenchers (for DNA)

 

Type of Modification
Position
Quenching Range
5’
3’
Int.
(nm)
 
X
  480-580 / 560-670
Dabcyl  
X
  400-550
IQ-500    
X
 

 

Black Hole Quencher-1

 
BHQ-1 is best suited for fluorophores that emit in the wavelength range from FAM to TAMRA.
 
Position
Synthesis Scale
Purification
 
0.04
0.2
1.0
15
Des
HPLC
PAGE
5’
  O O
O
 
   
3’
 
X
X
O
 
X
X
X = available/on stock
O = on request

Black Hole Quencher-2

 
BHQ-2 is best suited for fluorophores in the Cy3 to Cy5 wavelength range.
 
Position
Synthesis Scale
Purification
 
0.04
0.2
1.0
15
Des
HPLC
PAGE
5’
  O O
O
 
   
3’
 
X
X
O
 
X
X
X = available/on stock
O = on request

Dabcyl

 
Dabcyl is a relatively weak dark quencher and is best suited for use in molecular beacon probes where the hairpin places the quencher and fluorescent dye in close proximity.
 
Position
Synthesis Scale
Purification
 
0.04
0.2
1.0
15
Des
HPLC
PAGE
3’
 
X
X
O
 
X
X
X = available/on stock
O = on request

Internal Quencher IQ-500

 
Special quencher for double-quenched probes.
 
Position
Synthesis Scale
Purification
 
0.04
0.2
1.0
15
Des
HPLC
PAGE
Int
 
X
X
O
 
X
X
X = available/on stock
O = on request