Back to top
Amplicon Deep Sequencing
- Detect introduced or expected mutations
- Discover rare genomic variations with high confidence
Considerations before starting an amplicon deep sequencing project:
- Locus-/Amplicon length?
- Expected complexity/Sequencing depth?
- DNA amounts?
Let us guide you – from design to analysis
Example projects using amplicon deep sequencing:
- Taxonomic analysis with specific species focus (Barcode of Life studies)
- CRISPR/Cas and TALEN verifications and screenings
- Species Occurence detetction (eDNA)
- High throughput sanger sequencing alternative for short sequences
Applications related to amplicon deep sequencing:
- 16S/ITS Metagenomics
- CRISPR/Cas Sequencing
- Whole Exome Sequencing
The results produced by our analysis module help answer two main questions of sequencing a target region (e.g., a specific gene; input may be amplicons or long range PCRs).
- Which are the single nucleotide variations and small insertions/deletions in comparison to the reference sequence and which are the effects of these detected variations on the protein level? (see Table 1)
- What is the confidence of the detected variations and what are trustworthy thresholds to distinguish noise from putative variations? (see Figure 1 and 2)
- What is the consequence of a certain treatment compared to a group of controls? (complementary comparative dereplication analysis) (see Figure 3)
Figure 1: This figure depicts the read coverage of a target region.
Figure 2: This figure shows an analysis of possible thresholds to distinguish noise from putative variations.