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Certificate of Analysis (CoA)

 
 
A certificate of analysis (CoA) issued by Microsynth provides test results that show product specifications have been met.
 
 

Overview

 
Each oligo synthesized by Microsynth is delivered along with a technical datasheet. On this technical datasheet, following information is listed:
  • oligo name, oligo ID, nucleotide sequence, modifications, length, synthesis scale selected by customer
  • amount in OD measured by Microsynth
  • molecular weight, melting point, yield in nmol and µg calculated by Microsynth
  • volume for obtaining a 100 µM solution calculated by Microsynth (in case you order the oligo in dried format)
In case the technical datasheet does not meet your requested level of documentation, Microsynth offers various types of CoAs for an extra charge. The standard CoA (Type 1) contains the following information:
  • Appearance test by means of visual inspection
  • Solubility test by means of solubilization in buffer prior to lyophilization
  • Information about re-suspension of your oligo and the stability (shelf life at different storage conditions)
  • Identity check by MALDI-TOF mass analysis including the mass spectrum
  • Purity test by means of analytical HPLC including the chromatogram (if secondary structure is too strong, or oligo is >50 mer, we do not perform this analysis)
  • Purity test by means of analytical PAGE (raw data not included)
  • Proof checked and signed by our head of oligonucleotide department or the delegated representative
In addition to the standard datasheet the following Certificate of Analysis (CoA) can be requested while ordering oligonucleotides: 
Type Description
1 Certificate of Analysis (complete)
2 CoA without declaration of oligonucleotide stability
3 CoA without MALDI TOF
4 CoA without analytical HPLC
5 Only MALDI TOF spectra
6 Only HPLC
7 CoA for ASO or PTO oligos (complete)
 
If you want to see an example of our Standard CoA or its derivatives, please email us.
 

How to Order

 
If you want to order your oligonucleotide along with a Certificate of Analysis, please select the desired type of CoA under “Data Sheet” during the online ordering procedure.