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DNA Oligonucleotides

DNA Oligonucleotides
 
 
Microsynth uses proprietary synthesizer technologies to offer oligonucleotides of highest quality. Our state-of-the-art production facilities enable fast synthesis of standard primers, modified and functionalized oligonucleotides as well as (dual-) labelled probes. You benefit from a comprehensive variety of modifications, different quality levels and final preparation options.
 

Features and Benefits

 
 
Highest Quality
  • Stringent quality control (online Trityl monitoring and  MALDI-TOF MS or analytical PAGE)
  • Microsynth is a pioneer with a successful track record of 28 years
 
Fast
  • Synthesis scales up to 1 µmol are shipped the next day
  • Standard modified oligos are shipped within 3 days
 
Comprehensive Service Portfolio
  • Large range of synthesis scales (yields from nmol to mmol are available)
  • Broad range of 5’-, 3’- and internal modifications
  • 4 different purity levels
 
 
 
Cost Effective
  • Very competitive pricing for large quantity and/or prepaid orders
  • High guaranteed yields

 

Excellent Technical Support
  • Trained scientists with molecular biology background are happy to support you (from 8 am to 5 pm)!
 
User-friendly Online Ordering System
  • New and easy-to-use online portal with a series of helpful tools (e.g. order tracking & history, convenient search and re-order option)

 

Synthesis Scales

 
Microsynth is offering its DNA oligos at 5 different scales. For large scale synthesis please get in contact with our experts.
The available standard scales are Genomics, 0.04 µmol, 0.2 µmol, 1.0 µmol and 15 µmol.
As the yield and length restrictions depend also on the purification used please have a look at the tables below.
 
 
 
Desalted DNA Oligos
Synthesis scale1 Length Restriction Guaranteed Yield2 Average Yield Production Time [wd]
[OD260] [nmol]3 [OD260]2 [nmol]3
Genomics 13 - 60 2 10 8 40 1
0.04 µmol 13 - 80 3 15 9 45 1
0.2 µmol 6 - 1504 10 50 20 100 1
1.0 µmol 6 - 80 50 250 80 400 1
15 µmol 13 -60 700 3'500 1'000 5'000 2
 
 
HPLC Purified DNA Oligos 
Synthesis scale1 Length Restriction Guaranteed Yield2 Average Yield Production Time [wd]
[OD260] [nmol]3 [OD260]2 [nmol]3
Genomics not available
0.04 µmol <51 1 5 4 20 2
0.2 µmol 3 15 7 35 2
1.0 µmol 15 75 26 130 2
15 µmol 300 1'500 500 2'500 3
 
 
HPLC Purified & Dialysed DNA Oligos 
Synthesis scale1 Length Restriction Guaranteed Yield2 Average Yield Production Time [wd]
[OD260] [nmol]3 [OD260]2 [nmol]3
Genomics not available
0.04 µmol not available
0.2 µmol <51 3 15 6 30 3
1.0 µmol 15 75 20 100 3
15 µmol 200 1'000 400 2'000 4
 
 
PAGE Purified DNA Oligos
Synthesis scale1 Length Restriction Guaranteed Yield2 Average Yield Production Time [wd]
[OD260] [nmol]3 [OD260]2 [nmol]3
Genomics not available
0.04 µmol 13 - 80 0.5 2.5 6 30 2
0.2 µmol

8 - 80

81 - 1504

2

0.5

10

2.5

8

4

40

20

 2
1.0 µmol 8 - 80 7 35 20 100 2
15 µmol not available
 
1 The synthesis scale represents the initial amount of 3' bases (starting material).
2 Our guaranteed and average yields are measured in OD and are valid only for unmodified oligos >20mer.
3 Yields indicated in nmol represent an example calculation for a 20mer. For this calculation the following rule of thumb equation was applied: nmol of oligo = OD x 100/length of oligo. Please note that this calculation is based on sequences with virtually homogenous distribution of the 4 DNA bases; it may vary for sequences with high GC contents >70% etc.
4 Oligos longer than 150 DNA bases on request (we would like to discuss the proposed experiment/application with you beforehand in order to guarantee the best possible outcome)

Modifications

 

Microsynth offers a wide variety of modifications (5’, 3’ and internal) for DNA oligonucleotides. To give you a better overview, they have been categorized as: 

To learn more about the key specifications of a modification, please look at Modifications.

 
Important - Please Note!
Microsynth is continuously expanding its portfolio of dyes which can be used as cost-effective alternatives to patent-protected dyes.
Examples are the following: 

Yakima Yellow or ATTO 532 for VIC™

ATTO550 for NED™

ATTO565 for PET™

Dyomics630 for LIZ™

LI-COR Sequencer compatible dyes Dy681/781 as alternatives for IRDyes® 700/80

If your desired modification is not listed please contact us for availability.

How to Order

 
  • Enter our webshop
  • Click on DNA in the "DNA/RNA Synthesis" domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel Template (can be downloaded during ordering )
  • Follow the further instructions

 

To obtain information about our prices, please login to our webshop and download the current price list or ask your sales manager for a specific quote (attractive discounts are available if you plan to order more regularly from Microsynth or if you have a volume order).