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Hydration

 
 
Every Microsynth oligo is deprotected and desalted to remove small molecule impurities. Your oligos are further quantified twice by UV spectrophotometry to eventually provide an accurate measure of yield (in OD, ug and nmol). For convenience, solutions at 100 µM concentration are most often prepared and used as freezer stocks (which should be thawed infrequently).

At Microsynth you can specify whether you want to receive your oligos dried down or hydrated (ready-to-use) at standard 100 uM or your dedicated concentration.

Overview

 

DNA/RNA Oligonucleotides

Oligos are usually shipped in dry form. The dried DNA pellet becomes dislodged from the bottom of the tube during shipping and it can easily fly out of the tube when first opened, particularly as electrostatic attraction is present. For this reason: Always briefly centrifuge your oligos before opening for the first time, if you order oligos in dried format.

If you want your oligos already delivered in liquid format1 at the commonly used 100 µM concentration (100 nmol/ml), you can easily select this option at our online shop for a small surcharge. Should you prefer to receive your oligos solubilized at a specific concentration and/or in a specific buffer, let us know and we will deliver according to your specifcations.

Special case: DNA oligos in plates:

Oligos in 96-well plates are often shipped in liquid format to avoid the laborious process of solubilization at customer side. Microsynth offers this option at very attractive prices or even for free in case of larger oligo numbers. Further, the delivery of replica plates (e.g. at 10 µM, 50 µM) along with the master plate (100 µM) is possible for a small surcharge.

 

siRNA

Our siRNAs are usually delivered ready-to-use: fully deprotected, sense and antisense strand already annealed and in liquid format2 at 40 µM concentration. Should you prefer to receive your siRNA in dried format or solubilized with a specific concentration/buffer, let us know.

DNA and RNA oligos are solved in water
siRNA is annealed in 10 mM Tris, pH 7.5, 20 mM NaCl

How to Order

 

If you want to order your oligo in liquid format at 100 µM concentration (100 nmol/ml), please select "100 nmol/ml" under "Physical condition" when specifying your order in our online shop. In case you want your oligos delivered at other concentrations, let us know and we will instruct you how to proceed.