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Resequencing of Eukaryotic Organisms
- Detect single nucleotide to large structural variations in your eukaryotic model organism
- Detect off target mutations of your CRISPR/Cas9 gene editing experiment
Considerations before starting a eukaryotic resequencing project:
- Is there a suitable reference genome?
- Optimal sequencing depth?
- Aim of the study versus limits of a reference transcriptome?
- Read length?
Let us guide you – from design to analysis
Example projects using eukaryotic resequencing:
- Detection of mutations aquired by cancer cells – from SNPs to large structural variations
- Insertion site detection
- Mutation verification, ruling out off-target mutations
- Genetic modifications in breeding studies
Applications related to eukaryotic resequencing:
- Microbial Resequencing and profiling
- RNA Sequencing
- Whole exome resequencing
- Which are the single nucleotide variations and small insertions/deletions in comparison to the reference genome and which are the effects of these detected variations on the protein level? (see Table 1)
- Which are the structural variations in comparison to the reference genome? (see Table 2)
- Which are the copy number variations in comparison to the reference genome (or to a reference sample)? (see Figure 1)
Table 1: This detail of a result table shows detected single nucleotide variations, small insertions and deletions and their annotation.
Table 2: This detail of a result table lists putative structural variations.
Figure 1: This figure depicts a possible copy number variation in relation to a reference sample.