Full-Length Plasmid Sequencing

Introduction 

Did you ever waste your time because the plasmid you worked with was not the plasmid you thought it was? Misidentified or mutations in plasmids can be a source of ongoing frustration and can hamper the progress of your research. Microsynth provides a fast and cost-effective Illumina-based full-length plasmid sequencing service including assembling/bioinformatics. Therefore, let us sequence your plasmids to make your research more reliable.

Your Benefits Include

  • Full-length characterization of unknown plasmids
  • Full-length validation of known plasmids (requires reference sequence)
  • Large bulk of sequencing data per plasmid
  • Cost-effective NGS sequencing including deep sequencing coverage and highest possible data quality
  • Fast turnaround time (1 to 3 weeks)

Routine Workflow

  • Plasmid Isolation (either done by customer or outsourced to Microsynth)
  • Library Preparation (shotgun libraries will be used)
  • Next Generation Sequencing (paired-end sequencing on the MiSeq system generating 2 Mb raw data using 250 bp read length mode or longer)
  • Bioinformatics Analysis (filtering of host DNA if reference sequence will be provided andde novo assembly of the plasmid)
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Contact Form
Interested to discuss your NGS project with an expert or to receive an offer? Then, please fill in our NGS contact form

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