FAQ

Product & Services

What is the difference between Barcode Economy Run Service and Economy Run Service?

The Barcode Economy Run Service is Microsynth‘s most demanded standard sequencing service for plasmids and PCR products in 1.5 ml tubes. It offers the best price-performance ratio due to its prepayment structure (minimally 50 barcode labels corresponding to 50 sequencing reactions have to be purchased in advance).

Microsynth’s Economy Run Service shows essentially the same service features as the Barcode Economy Run Service, except that customers will be invoiced after using the service.

When should I use Microsynth’s Premium Run Service?

The Premium Run Service is Microsynth‘s premium sequencing service in 1.5 ml tubes offering a broad spectrum of additional services (e.g. DNA concentration measurement, special treatment protocols for difficult sequences, manual sequence editing) and troubleshooting protocols (e.g. alternate sequencing primer design, one-drop sequencing in case of low DNA amounts).

Generally speaking, it is most suitable for customers expecting the best possible outcome along with superior service.

Does Microsynth offer free shipment of my sequencing samples?

Microsynth and its subsidiaries (Microsynth Austria GmbH and Seqlab GmbH) operate three different sequencing labs in Balgach (Switzerland), Vienna (Austria) and Göttingen (Germany). Within the last couple of year, a growing net of free sample collection points has been installed around these 3 key sequencing centers. Please contact us and we will inform you if you can profit from a cost-free sample pick-up service within your immediate environment.

If you enter to our webshop the nearest points combined with the detailed descriptions are listed under "View collection points".

How long do you store my DNA samples and primers?

o Barcode Economy Run or Economy Run Service samples: 5 working days
o Premium Run Samples: 3 months
o High-Throughput sample plates: 1 months

o Your specific sequencing primers will be kept at our sequencing lab for at least 6 months (or for 12 months in case you have added them to your “Custom Primer List”).

Can I get back my specific primer(s) following sequencing?

On request, Microsynth will ship your specific sequencing primer(s) back to your or any other desired address.

Is it possible to get back the isolated plasmid(s) after sequencing?

Yes. In case you have high-copy plasmids and only one antibiotic resistance per plate, we will ship you back the isolated plasmids (~0.5-1.0 ug in Tris-HCl pH 8,5 or lyophilized) free of charge.

When filling in the order form, just place a note in the comment field.

Which sequencing primers do you offer/add free of charge?

Click here in order to be linked to the “Hints and Tips” area where you will find a list of sequencing primers where you can select from.
Please note: Microsynth’s webshop allows you to implement a customized primer list. In case you frequently use more specific sequencing primers, just send them to Microsynth and update your customized primer list. Since Microsynth operates a well-recognized oligo production facility, you can also outsource design and/or synthesis of your sequencing primers.

Order Related Questions

How do I have to prepare and pack my samples?

Dependent on the selected service type, your samples have to fulfill different requirements. For following services, Microsynth provides user guides containing a lot of helpful information:

o Barcode Economy Run Service
o Economy Run Service
o Premium Run Service
o Barcode High-Throughput Service
o High-Throughput Service

Please click here in order to be transferred to the “Download Area” where you can download the required user guide; or click here in order to be linked to the “Hints and Tips” area where you will also find useful information about sample requirements etc.

How can I review my previous orders?

•    Enter our webshop
•    Click on Select Services in the “DNA Sequencing” menu
•    Click on History menu bar at the top

Microsynth claims to repeat failed sequencing reactions for its standard sequencing services in 1.5 ml tubes (Economy and Barcode Economy Run) free of charge. How does it work?

In general, failed sequencing reactions will be automatically repeated in the following mode: In a first attempt one sample will be repeated in order to check the potential for further improvement. For a repetition we modify our treatment (e.g. we try the GC-rich treatment if you haven’t ordered it before or we leave it away if the first attempt was done with it). If the repetition yields in a better sequencing result, other samples of your order will get resequenced, too. In addition, it is always possible to ask for specific repetitions of failed reactions in a customized manner (best done by phone or e-mail). But be aware, that we keep Economy and Barcode Economy Run samples only for 4 working days.

General Technical Questions

What are the critical requirements for sequencing primers?

Click here in order to be linked to the “Hints and Tips” area where you will find a lot of useful information about sequencing primers.

How can I open the ab1 files?

Microsynth provides the sequencing results in text format (FASTA files) and in chromatogram format (ab1 files). In order to open the ab1 files you need to download one of the software tools which are provided in the Software and Links area.

Can I sequence small parts of mouse, human or any other genomes directly with Sanger sequencing?

Direct sequencing via the Sanger approach is possible for genomes < 5Mb. However, whenever feasible, we recommend you to amplify the region of interest by PCR and to send us the PCR product(s) for sequencing.

What is important for sequencing PCR products?

In order to obtain reliable sequencing results, impurities such as dNTPs, PCR primers etc. must be eliminated from the PCR product before sequencing. Furthermore, it is important to quantify the purified PCR product. This is easily accomplished by performing agarose gel electrophoresis of your PCR product and comparing the band for your PCR product with standard bands of defined concentrations. We do not recommend quantification approaches based on spectrophotometric analysis techniques. For successful sequencing, PCR products must be single-banded on the agarose gel. If this is not the case, you should recover the correct band by gel extraction.



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