Easy-to-Use Cell Line Authentication Service for Human, Mouse and Other Species
Why to Test Cell Lines?
It is a fact that cross-contamination and misidentification of mammalian cell cultures is widespread. An incredible high number of 15-20% of all cell line based biomedical research is affected by misidentified cell lines. Therefore, establishing a cell line’s identity through STR profiling is essential to conducting valid and re-producible research. Moreover, more and more journals (not only the high-impact ones!) are requesting the authentication of cell lines as prerequisite for acceptance of manuscripts.
Microsynth has over 10 years of experience in genotyping and offers an easy-to-use service for human and mouse cell lines. Rely on our experience and make your research reliable!
Main Service Features and Benefits:
- Database comparison of the DNA profile (ATTC, DSMZ)
- Mycoplasma contamination testing of cell culture supernatant
How it Works?
- Collect 1.0-5.0 Mio cells and wash the cell pellet twice in PBS or another appropriate buffer. Resuspense cell pellet in 0.5 ml of 70-90% ethanol and transfer to 1.5 ml screw cap tube. If you want to send us isolated DNA, please provide ≥30 μl gDNA in Tris or low-EDTA buffer (10 mM Tris, 0.1 mM EDTA). For mycoplasma testing please send 100 μl of the culture supernatant directly.
- Download and fill the order form (see under Related Downloads). Send a copy to firstname.lastname@example.org.
- Print the order form and put it together with the samples in a plastic bag or envelope. Drop the samples in the next Microsynth or Seqlab collection box. To find the closest collection point please login the webshop at www.microsynth.ch and click “Options & Preferences” under DNA Sequencing and find details under “Collection Points”.
- Microsynth will confirm the receipt of your samples and ship back the analysis results within one week.
To obtain information about our prices, please login to our webshop and download the current price list (see page 10 for cell line authentication services). New customers will get an introductory discount of 50% for the first cell line.
- ATCC SDO Workgroup ASN-0002 (2010). Cell line misidentification: the beginning of the end. Nature Rev. Cancer 10:441–448.
- Barallon, R. et al (2010). Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues. In Vitro Cell.Dev.Biol.— Animal 46:727–732
- Chatterjee, R. (2007). Cases of Mistaken Identity. Science; 315 no. 5814:928-931
- Almeida, J. et al (2013). Mouse cell line authentication. Cytotechnology 66: 133-147.
- Yu, M. et al. (2015). A resource for cell line authentication, annotation and quality control. Nature 520:307-311