siRNA for Gene Silencing


Overview
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 siRNA, or small interfering ribonucleic acid, is a type of RNA that is involved in a number of biological processes, most notably RNA interference. RNA interference is a regulatory process that is used to control and limit the expression of specific genes. While most RNA is single stranded, siRNA is made up of two complementary strands of RNA nucleotides similar to those in DNA. Usually siRNA molecules are 21-23 nucleotide double-stranded RNA (dsRNA) duplexes with symmetric 2-3 nucleotide 3’ overhangs.

Main Product Features and Benefits:

  • High quality: all siRNAs are MALDI-TOF controlled before shipment
  • Guaranteed yields: high quantity delivered (actual delivered amount significantly higher than minimal guaranteed yield)
  • Ready to use: fully deprotected, siRNAs already annealed and ready to use (40 µM)
  • Fast delivery:
    • Shipment within 24 hours (valid only for single orders; orders must be placed before 10 AM, and a short note should reach us by telephone)
    • Shipment within 48 hours (valid only for single orders; for volume orders please request information on delivery time) 
  • Free of charge: free siRNA design service available
  • siRNA Design Tool: By using our online siRNA design tool you can design your siRNAs on your own (our design tool represents a customized tool that combines published design criteria with valuable experiences made by Microsynth). To use our siRNA design tool please login first to our webshop and choose siRNA > siRNA Design Tool.
  • Additional services:
    • Modified siRNAs are available on request
    • Guaranteed knock down: if you use our free siRNA design service and let us produce ≥ 3 siRNAs, we guarantee that at least one of the synthesized siRNAs will reduce mRNA levels by at least 70%  

siRNA Duplexes (2 x 21mers and Longer) >>to the top

 Microsynth supplies classical siRNA duplexes (2x21-mers) and longer duplexes up to 27-mers with 3 different levels of purity:

  • Desalted siRNA
  • HPLC purified siRNA
  • PAGE purified siRNA

To learn more about product characteristics, please see the table below. To obtain information about our prices, please login to our webshop and download the current price list.


Product Characteristics:

Synthesis scale1

Desalted

HPLC

PAGE

OD2602

[nmol]2

OD2602

[nmol]2

[OD260] 2

[nmol]2

 

0.04 µmol

9

21

4

8

not available

0.2 µmol

16

35

7

16

2

5

Classical siRNA duplexes (2x21-mers) have unit prices; if you need longer duplexes, each additional RNA base will be priced accordingly. Modified siRNAs are available upon request (please have a look within the section RNA Oligos – Modified in order to see the range of modifications Microsynth offers as a standard service.


1 The synthesis scale represents the initial amount of 3' bases (starting material).

2 Yields indicated represent guaranteed yields and apply to a 2x21mer; Calculation: 1 OD = ~2.5 nmol (please note that this calculation is based on sequences with virtually homogenous distribution of the 4 RNA bases)

 

How to Order?

  • Enter our webshop
  • Click on siRNA in the "Oligonucleotide" domain
  • Select Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select siRNA Design Tool in case you first need to design your siRNA

Design Service for siRNAs – Free of Charge >>to the top

 
 

Rely on our vast experience in the area of siRNA synthesis and let us design your siRNAs including negative controls.


How to Proceed:

  • Contact us by e-mail and
  • Provide us with the accession number or alternatively with the mRNA sequence of your target gene
  • We then provide you with the sequence information of your desired siRNA
  • For siRNAs to be used as negative controls, we perform scrambling of your siRNA sequence and do a BLAST to theoretically exclude any interference with known mRNA sequences
  • Optional: You order ≥3 siRNAs and we guarantee that at least one of the synthesized siRNAs will reduce mRNA levels by at least 70%

Conditions:

  • Service is limited to 5 genes per customer and day
  • If you do not achieve the guaranteed 70% knock down, we design/synthesize additional 3 siRNAs for you. However, you need to show us that your experiment was performed according to the following parameters:
    • Delivery efficiency of siRNA: >90%
    • Effective concentration applied: 100 nM
    • Incubation: at least 48 hours
    • Positive control: included                      

siRNA Design Tool:

If you want to design your siRNA on your own, you can use our siRNA design tool (our design tool represents a customized tool that combines published design criteria with valuable experiences made by Microsynth). To use our design tool please login first to our webshop and choose siRNA > siRNA Design Tool. You will find some useful information about design criteria in the section “Hints and Tips” under Design Guidelines for siRNAs.

 


Pre-synthesized Control siRNAs >>to the top

 
 

Product Characteristics:

siRNAs

Other Specifications

Price

Lamin A/C - CUGGACUUCCAGAAGAACAtt

  • desalted

  • delivered as 4 nmol aliquots

  • sequences are published and are not known to show any off target effects [1,2]

CHF 75.00 / EUR 48.39

GFP - GCAGCACGACUUCUUCAAGtt

Luciferase - CGUACGCGGAAUACUUCGAtt

Neg. control - AGGUAGUGUAAUCGCCUUGtt

1 Trincheri, N. F., Nicotra, G., Follo, C., Castino, R., & Isidoro, C (2006). Resveratrol induces cell death in colorectal cancer cells by a novel pathway involving lysosomal cathepsin D. Carcinogenesis, 28(5), 922-931. doi:10.1093/carcin/bgl223

 

2 Talotta, F., Mega, T., Bossis, G., Casalino, L., Basbous, J., Jariel-Encontre, I., u. a (2010). Heterodimerization with Fra-1 cooperates with the ERK pathway to stabilize c-Jun in response to the RAS oncoprotein. Oncogene, 29(33), 4732-4740. doi:10.1038/onc.2010.211


 

How to Order?

 

You need only control siRNA (option 1):

  • Write an e-mail containing your account information (name, delivery address, login). Indicate the name and number of the control siRNA(s) you would like to order.

You want to order your control siRNA along with other siRNAs (option 2):

  • Enter our webshop
  • Click on siRNA in the "Oligonucleotide" domain
  • Select Normal Entry in order to type or copy/paste the desired sequence information etc. for your siRNA. In the field "special comment" you can specify the name and number of the required control siRNAs.

Scrambled siRNAs (Customized Negative Control siRNAs) >>to the top

 
 

Product Characteristics:

 

A scrambled siRNA is used as negative control to discount any changes to the gene expression profile that may result from the siRNA delivery method. This negative control siRNA is generated by scrambling your siRNA sequence, but keeping the nucleotide composition the same.

How to Order?

  • Write an e-mail and provide us with the accession number or alternatively with the mRNA sequence of your target gene. We perform scrambling of your siRNA sequence and do a BLAST to theoretically exclude any interference with known mRNA sequences. Then we provide you with the siRNA sequence.
  • Enter our webshop
  • Click on siRNA in the "Oligonucleotide" domain
  • Select Normal Entry in order to type or copy/paste the obtained sequence information for your control siRNA.
 





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Questions?
Write an e-mail or call us at +41-71-722 83 33

Related Downloads
siRNAs.pdf
Gives an overview about Microsynth's fast synthesis service for high-quality siRNAs


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