Applications Using Microsynth NextGen Sequencing Technologies

454 from Roche, SOLiD 5500xl from Life Technologies or Sanger 3730xl from ABI?

Many years’ experience in DNA sequencing allows us to offer the following broad spectrum of up-to-date sequencing applications:

• Genome: De Novo Shotgun and/or Paired-end Sequencing
• Genome: Re-sequencing/Targeted Sequencing/Human Genome and Exome Sequencing
  
• Transcriptome: Whole Transcriptome & Small RNA Sequencing
  
• Metagenome Sequencing
  
• Amplicon Sequencing
  

The following table is a guideline to choose the appropriate sequencing technology for your sequencing application/project. The recommendation is based on an optimal cost-benefit ratio. For many sequencing projects, a hybrid approach using several sequencing technologies is the best procedure.

• Backbone structure with the 454 technology: Library preparation – shotgun sequencing for the contigs generation, –paired-end sequencing (3kb and 8 kb) for contigs orientation in scaffold. Sequencing of eukaryotic, prokaryotic and virus samples on full or partial run.
• Bioinformatics: Electronic assembling
• Finishing and gap closure with Sanger technology

• Comparative re-sequencing of multiple whole genomes using SOLiD technology for SNPs, insertions, deletions analyses. The high accuracy of SOLiD enables variant detection even with a lower coverage.
• Enrichment technology (Agilent, SureSelect) for whole exome sequencing
• Customized enrichment (Agilent, SureSelect) for in-depth analyses of specific regions on the genome in a large number of samples
• Genome-wide DNA methylation pattern analysis
• Bioinformatics: Electronic assembling and/or mapping to genome, sample sorting, SNP analyses

• Reference full transcriptome of normalized cDNA library using the advantages of the read length from 454 technology
• Comparative transcriptome analyses (expression profile, splice variant detection) and small non-coding RNA sequencing on high-capacity SOLiD technology
• Bioinformatics: Electronic assembling and/or mapping to the transcriptome, sample sorting, expression levels

• Shotgun sequencing of the whole bacterial community DNA. The long reads obtained with 454 technology enable diversity, metabolic and microbial abundance studies of the metagenomes of interest.
• Deep sequencing: For example, analysis of the 16s rDNA of a bacterial community
• Bioinformatics: Electronic assembling and/or mapping, sample sorting

• Detection of genetic variation with ultra-deep sequencing of PCR products
• Bioinformatics: Samples sorting, mapping to reference