Practical Information
Here you will find practical information for the realization of your sequencing project.
| Shipment of Samples | >>to the top |
Please ship your samples to the following address:
Microsynth AG
Next Generation Sequencing
Schützenstrasse 15
9436 Balgach
Switzerland
We recommend shipping the samples frozen on dry ice.
| Preparation of Samples | >>to the top |
Buffer Recommendations for 454 Sequencing:
| Type of Sequencing | Buffer |
| Shotgun and amplicon sequencing |
TE buffer, water or 10 mM Tris-HCl buffer (pH 7.5 – 8.5) |
| Paired-end sequencing | 10 mM Tris-HCl buffer (pH 7.5 – 8.5) |
Buffer Recommendations for SOLiD Sequencing:
| Type of Template | Buffer |
| DNA |
Low TE, water, 10 mM Tris-HCl buffer (pH 7.5 – 8.5) |
| RNA | 10 mM Tris-HCl buffer (pH 7) |
| Type of Library | Amount (µg) | Concentration (ng/µl) |
| Shotgun | >2 | >50 |
| 3kb paired end | >15 | >100 |
| 8kb paired end |
>25 |
>200 |
| Amplicon | >0.2 | >5 |
| Total RNA | >10 | >100 |
| Poly(A)RNA | >2 | >100 |
Sample Amounts for SOLiD Sequencing:
| Type of Library | Amount (µg) | Concentration (ng/µl) |
| Fragment/paired end | >5 | >100 |
| Mate paired | >20 | >100 |
| Targeted enrichment | >10 | >100 |
| Total RNA for whole transcriptome analysis |
>10 | >100 |
| Total RNA for small RNA analysis | >5 | >100 |
| rRNA-depleted RNA or poly(A) RNA | >1 | >100 |
DNA/RNA Quantification
DNA/RNA quantification is recommended to be done by a fluorometric method, e.g. PicoGreen®, RiboGreen®, Qubit®, etc.
Sample Storage at Microsynth
Samples as well as the processed libraries will be stored at Microsynth for at least 6 months.
Submission of the Samples
Please use the "sample submission form" (can be downloaded under “Related Downloads” in the right column)
| Data | >>to the top |
Data Storage at Microsynth:
| Guidelines for Amplicon Projects | >>to the top |
The PCR product should have a length between 300 and 500 bases (including the fusion primers). Within one pool all the PCR products should optimally not differ more than 50 bases in length. If the difference in length is higher, the sequencing will be affected by a decrease in the run/region capacity. More specifically, due to the approximately 400 bp read length of the Genome Sequencer FLX System, the distance between the two template-specific primers must be given careful consideration if the "inserts" need to be sequenced entirely.
For information about uni- and bidirectional amplicon sequencing, please click "Fusion Primers for Amplicon Sequencing" under Related Links (see the right column on top of page)
