Primer Walking Services

Do you need the complete sequence of a plasmid insert or any other DNA template which is longer than the average read length of 800 bases? Then we recommend using one of the following primer walking services:

• Non-assembled Primer Walking Service (Single-stranded and Double-stranded)
  
• Assembled Primer Walking Service (Single-stranded and Double-stranded) 
• Primer Walking Service for Sequence Verification (single-stranded and Double-stranded) 

To learn more about this type of sequencing service, please see the various sections below. To obtain information about pricing, please enter our webshop and download the current price list.

 

 

Non-assembled Primer Walking Service (Single-stranded and Double-stranded)

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In general, your DNA template is sequenced by a series of primer walking reactions. Since we are able to produce the sequencing primers in-house, we achieve a relatively high sequencing speed. On average, we perform one sequencing reaction per day. If you choose the double-stranded service, both strands are sequenced to confirm every established base. Our double-stranded sequencing service produces a sequence accuracy of 99.999% for standard templates, i.e. less than one error in 100’000 base pairs of sequence (publication quality).

 

Upon sequencing your template, you will receive the single sequences. Final assembly will have to be done at your end.

 

Assembled Primer Walking Service (Single-stranded and Double-stranded)

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In general, your DNA template is sequenced by a series of primer walking reactions. Since we are able to produce the sequencing primers in-house, we achieve a relatively high sequencing speed. On average, we perform one sequencing reaction per day. If you choose the double-stranded service, both strands are sequenced to confirm every established base. Our double-stranded sequencing service produces a sequence accuracy of 99.999% for standard templates, i.e. less than one error in 100’000 base pairs of sequence (publication quality).

 

Upon sequencing your template, assembly of the single sequences will be completed quickly and accurately at Microsynth. You will receive an electronic and printed version of the aligned sequence. 

 

 

Primer Walking Service for Sequence Verification (single-stranded and double-stranded)

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In general, your DNA template is sequenced by a series of primer walking reactions. Since we are able to produce the sequencing primers in-house, we achieve a relatively high sequencing speed. On average, we perform one sequencing reaction per day. If you choose the double-stranded service, both strands are sequenced to confirm every established base. Our double-stranded sequencing service produces a sequence accuracy of 99.999% for standard templates, i.e. less than one error in 100’000 base pairs of sequence (publication quality).

 

Upon sequencing your template, we will do first assembly of the single sequences and then verify the obtained sequence against your original sequence. You will receive an electronic and printed version of the aligned sequence as well as a description of discrepancies.

 

Main Service Features and Benefits for all Primer Walking Services

Parameter	Non-assembled		Assembled		Sequence Verification
	SS*	DS**	SS	DS	SS	DS
Fast in-house design and synthesis of primers	x	x	x	x	x	x
Delivery of synthesized sequencing primers on request	x	x	x	x	x	x
Read length per run: up to 1100 bases	x	x	x	x	x	x
Fast sequencing with 1 run per day on average***	x	x	x	x	x	x
Editing of sequences	x	x	x	x	x	x
Delivery of electronic files such as project data sheet inclu-ding sequencing strategy, text files and chromatograms for each single reaction	x	x	x	x	x	x
Delivery of chromatograms for each single reaction	x	x	x	x	x	x
Accurate assembly of single sequences			x	x	x	x
Verification of an assembled sequence against its original sequence					x	x

* SS stands for single-stranded.
** DS stands for double-stranded.
*** For instance, a 3 kb DNA sequence can be decoded within 5 days.

 

How to Order?

• Enter our webshop
• Click on Select Services in the “DNA Sequencing” menu
• Click on Order Form in the “Primer Walking Service” domain and follow the further instructions