Clone & Sequence Service
Are you looking for a turnkey project partner in sequencing projects?
PCR products can be sequenced directly. However, the chromatogram corresponds to an average molecule. The information of the individual molecules is not available, unless these are cloned into a sequencing vector and sequenced from there. Depending on the origin of the PCR, numerous scientific questions can be solved, for example identification of microorganisms and relative numbers by their 16-S-rDNA or determination of individual mutations/rates. Since such projects are highly individual, we offer customized solutions allowing you to outsource as much as possible and to focus on the real scientific questions.
The service described here starts with your PCR product:
- Quality check of the PCR Product
- Agarose gel extraction
- Cloning into a sequencing vector
- Plating of individual clones
- Clone picking
- DNA extraction
- Sequencing of individual clones usually 10, 100 or 1000, depending on the project, and the required statistics.
We also offer services upstream from the PCR product such as DNA/RNA extraction from different species, PCR establishment, PCR amplification and services downstream from the obtained sequences such as mutation reports and answers to bioinformatics questions.
Technical, Pricing and Ordering Information?To obtain more information about this service and its prices or to place an order, please contact our experts.