Barcode Economy Run Service

Do you have a relatively high need for sequencing of routine samples such as PCR products or plasmids? Then we recommend our Barcode Economy Run Service.

 

To learn more about this type of sequencing service including pricing, please see the various sections below. To obtain information about detailed prices, please login to our webshop and download the current price list.

 

Main Service Features and Benefits:

  • Read length per run: up to 1100 bases
  • Standard primers and specific primers will be added free of charge
  • Failed reactions will be repeated free of charge1
  • Convenient service
    • Easy ordering and overnight in-house production of specific sequencing primers
    • Pre-paid barcode labels can be purchased in batches of 50, 100, 250 and 500, or 1000. They have no expiration date and can be used by multiple researchers.
    • Each of our green barcode labels allows unambiguous sample identification
  • User-friendly and cost-free sample shipment: Just use either Microsynth’s pick-up service or request our pre-paid and pre-addressed envelopes for cost-free shipment via post mail.
  • Rapid turnaround time: sequencing time in the laboratory <24h
  • Direct access to support by experienced academic staff (no waiting on a recorded-message hotline)
  • Additional services:
    • PCR purification available (additional fee is applicable)
    • Specific treatment for GC-rich samples (free of charge!)2

Pricing

Prices go down to CHF 7.00 / EUR 5.00 per reaction or barcode label (discount is dependent on purchase quantities)   

Sample Requirements

General Information:

DNA samples and sequencing primers can be sent pre-mixed (within one tube) or separate (different tubes). Each DNA sample should have a volume of 12 µl. In case you wish that Microsynth adds your sequencing primer, please make sure that you send us sufficient amount of your primer solution (minimally 20 µl of a 10 µM solution; in case you want to store your primer at our lab, consider that each sequencing reaction consumes at least 3 µl). DNA samples and primers for sequencing reactions are best dissolved in pure water. Alternatively, 10 mM Tris-HCl (pH 8) or 10 mM Tris-HCl (pH 8) with a maximum of 0.01 mM EDTA can be used for a better long term DNA stability. Standard TE-buffer is not suitable, because higher EDTA concentrations inhibit polymerase activity. Templates are stored for 1 week whereas primers will be kept at Microsynth for 1 year.

 

Sample Amounts & Concentrations

DNA Template

Concentration

Effective Amount (in 12 µl) Pipetting Scheme for Pre-mixed Option

Plasmid                                     

60-100 ng/µl3

720-1200 ng 12 µl DNA template solution  + 3 µl sequencing primer solution
 

PCR

   18 ng per 100 bases in a volume of 12 µl

PCR (200bp)

3.0 ng/µl

36 ng
PCR (300bp) 4.5 ng/µl 54 ng
PCR (400bp) 6.0 ng/µl 72 ng
PCR (>400bp) etc. etc.
Primer (pre-mixed)
Primer (separate)4
2 pmol/µl
10 pmol/µl = 10 µM
30 pmol
-

Important – Please Note!

Free pick-up service: Our customers in Switzerland (Basel, Bern, Lausanne and Zurich) and Austria (Graz, Innsbruck and Vienna) may benefit from our free pick-up service. In order to determine whether there is a Microsynth collection box in your immediate vicinity, please register/ login and see under “View Collection Points”. For all customers without a Microsynth collection box close by, Microsynth offers convenient and cost-free shipment of samples via its pre-paid and pre-addressed envelopes.
Overnight sequencing: Customers in Switzerland and Austria (Graz and Vienna) with direct access to a Microsynth collection box may benefit from Microsynth’s overnight sequencing service. Samples are sequenced overnight and results are sent by 10 am the following business day. It is important to note that specific sequencing primers have to be premixed with the templates when utilizing this type of sequencing service.

 

How to Order?

a) To order a batch of barcode labels:

  • Enter our webshop
  • Click on Select Services in the “DNA Sequencing” menu
  • Click on Order Barcode Labels in the “Barcode Economy Run” domain and follow the further instructions

b) To submit barcode-labeled tubes for sequencing:

  • Enter our webshop
  • Click on Select Services in the “DNA Sequencing” menu
  • Click on Order Form in the “Barcode Economy Run” domain and follow the further instructions


1 In general failed sequencing reactions will be automatically repeated in the following mode: In a first attempt one sample will be repeated in order to check the potential for further improvement. For a repetition we modify our  treatment (e.g. we try the GC-rich treatment if you haven’t ordered it before or we leave it away if the first attempt was done with it). If the repetition yields in a better sequencing result, other samples of your order will get resequenced, too. In addition, it is always possible to order specific repetitions of failed reactions in a customized manner (best done by phone or e-mail). But be aware, that we keep Barcode Economy Run samples only for 4 working days.
2
You are recommended to choose this option in case your sequence contains GC-rich sequences or hairpin structures. We then apply a protocol which has been developed specifically for GC-rich sequencing templates. Routine samples show best results with the standard protocol, whereas GC-rich samples show best results with this new protocol.

3 In order to yield good sequencing results, please adjust your DNA plasmid solution within the requested concentration range. Please consider that the optimal DNA concentration is 80 ng/µl.
4 Direct primer synthesis for sequencing possible.






Questions?
Write an e-mail or call us at +41-71-722 83 33



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