Human Cell Line Identification Service

Cross-contamination and misidentification of mammalian cell cultures is widespread, affecting an estimated 15–20% of the cell lines currently used in biomedical research. Several high-impact journals are already refusing to review submitted manuscripts unless they contain information on the authentication of cell lines used in a study, and in the near future similar restrictions are likely to be applied to grant applications as well [1-6].


Don’t take any chances: Let Microsynth characterize your cell lines.


You and Your Team Will …

  • save time and money
  • reduce the risk of inconsistent and non-reproducible findings
  • eliminate doubts on the quality of your data
  • facilitate peer review of your submissions
When to Test?
  • whenever a new cell line is established in or acquired by your laboratory
  • as a standard quality control measure (at regular intervals and prior to starting a new series of experiments)
  • before publication of research
  • whenever cell line performance is inconsistent or results are unexpected
  • to establish a genetic profile for a new or unique cell line

How Does it Work?

Cell lines can be authenticated by analyzing a number of highly polymorphic short tandem repeat (STR) loci dispersed throughout the genome. Microsynth will characterize your human cell line by analyzing the following set of STR markers (PowerPlex®16HS): D21S11, TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818, FGA, D8S1179, D3S1358, D18S51, Penta D, Penta E, Amelogenin (gender specific marker).


How to Test?

You send us:
 We send you:
Option 1:  50-100 µl of 1-2 ng/µl purified DNA  (A260/A280 ≥1.8) in Tris or low-EDTA TE (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0) STR Allele Report including all American Type Culture Collection (ATCC) standard markers for
cell line authentication
Option 2:  a cell pellet containing 1 to 5 x 106 human cells (please be aware: requires DNA isolation and will result in an additional charge)

Pricing and Ordering Information:

To obtain information about prices, please register/login and download our current price list or contact us. If you want to place an order please download the order form under “Related Downloads”, fill in accordingly and forward your samples along with the order form to:

                     Microsynth AG
                     Genotyping
                     Postfach 63
                     9436 Balgach

                     SWITZERLAND

 

Important: please ship your cell pellets on dry ice via a courier service. Make sure that you use enough dry ice.

 

References and Additional Information
Reference Literature:

  1. ATCC SDO Workgroup ASN-0002 (2010). Cell line misidentification: the beginning of the end. Nature Rev. Cancer 10:441–448.
  2. Barallon, R. et al (2010). Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues. In Vitro Cell.Dev.Biol.— Animal 46:727–732
  3. Dunham, J.H. et al (2009). Doing Good Science: Authenticating Cell Line Identity. Promega Notes 101:15–18
  4. http://grants.nih.gov/grants/guide/notice-files/not-od-08-017.html (Notice Regarding Authentication of Cultured Cell Lines, issued by NIH on November 28, 2007)
  5. Chatterjee, R. (2007). Cases of Mistaken Identity. Science; 315 no. 5814:928-931
  6. Masters, J.R. et al (2001). Short tandem repeat profiling provides an international reference standard for human cell lines. Proc. Natl. Acad. Sci. USA 98:8012–17.

Useful Links:

  1. http://www.lgcstandards-atcc.org/en/STR_Database.aspx

Via this link you will be carried to the American Type Culture Collection (ATCC) homepage with an updated list of reference standards for human cell lines. You have to register as a user.

 

 

 

 

 

 

 

 

 





 

Your Contact:
Write an e-mail to
or call him at
+41-71-726 10 00


Related Downloads
Purchase Order form.doc



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