Real-Time PCR Assays for Advanced Food Testingequest

Do you want to trace or quantify marker genes of animal specific, allergenic or transgenic background in food samples?

 

Main Product Features and Benefits:

  • Validated PCR assays (multiplex, quantitative, highly specific)
  • Ready-to-use kits with detailed SOP: just solubilize the primer/probe mixture in ddH2O and add your PCR Mastermix (not included)
  • High efficiency (assay with up to 5 markers in one PCR run!)
  • High quantity delivered: 1 kit contains 5 tubes with primer/probe sets for 5 x 20 PCR reactions (25 µl volume)
  • Excellent price/performance ratio

Potential Applications:

  • Food quality and safety testing
  • Allergen detection

Pricing, Ordering & Technical Information

To obtain information about pricing or to place an order please contact Dr. Vital wohlgensinger. If you are interested in more technical information, please contact Dr. Rene Köppel.

Product Characteristics

Allergen-Specific Kits:

Product Name Article Number Assay Type Marker 1 Marker 2 Marker
3
Marker 4 Marker 5 Re-
ferences
AllAll A 1200 Tetraplex Peanut Celery Soybean Hazelnut   [1, 16]
AllAll B 1202 Tetraplex Cow's milk
Almond Chicken egg Sesame   [2, 16]
AllAll G 1231 Tetraplex Lupine Almond Para Nut Sesame    
* brown and black mustard

Species-Specific Kits:
Product Name Article Number Assay Type Marker
1
Marker
 2
Marker
3
Marker
4
Marker
5
Re-
ferences
AllMeat 1204 Tetraplex Chicken Pork Beef Turkey
[4,5,6,16]
AllHorse 1206 Tetraplex Sheep Pork Beef Horse   [7,8,16,17]
AllMilk 1217 Tetraplex Cow Goat Sheep Water buffalo

[9]
AllPaté 1223 Pentaplex Chicken Duck Goose Turkey Pig [19]
AssignAnimal 1225 This SYB Green real-time PCR kit allows typification of different species in combination with Sanger DNA sequencing. Amplification is based on the conserved cytochrom B region of DNA from mammals, birds and fishes. [20]

GMO-Specific Kits:

Product Name Article Number Assay Type Marker
1
Marker
 2
Marker
3
Marker 4 Marker
5
Re-
ferences
AllGVOScB 1215 Pentaplex Nos- terminator 35S promoter Soy (lectin) Mais (mhmg) CaMV
[10]
AllGVOScC 1210 Tetraplex FMV promoter Bar gene CP2 / CP4 / EPSPS Pat gene   [11,16]
AllSoyA
1212 Pentaplex Roundup Ready Mon89788 Soybean Le1 gene Soy2704  A5547-127 [12,13,16]
AllMaize C 1227 Pentaplex Starlink CBH Mon863r T25 Maize(mhmg) Mon810 [10]
AllMaize D 1229 Pentaplex Bt11 Nk603 Ly038 Maize (mhmg) Bt176 [10]
AllSoyB 1235 Pentaplex DP305423-1 DP356043-5 Lectin CV127-9 Mon87701 [16]

Microorganism-Specific Kits:

Product Name Article Number Assay Type Marker
1
Marker
2
Marker
3
Marker
4
Marker
5
Re-
ferences
AllBakA IPC* 1219 Pentaplex E. coli Stx1 Stx2 intimin Salmonella (incl. Bongori) Listeria mono- cytogenes Campylo-bacter spp IPC pUC19
[14]
AllCamp 1221 Tetraplex Campylo-bacter jejuni Campylo-bacter lari Campylo-bacter coli IPC pUC19   [15]
AllColi 1233 Tetraplex E. coli spp E. coli eae/intimin E. coli Stx1 E. coli Stx2   [18]
* interlaboratory trial ongoing


Important - Please Note!

To run our assays successfully, you need an adequate real-time PCR mastermix (see Reference 16) and a real-time PCR thermocycler capable of measuring multiplex reactions at the required wavelengths.
All Pentaplex assays are also available with the dye ATTO 425 instead of Dyomics 681, in case your real-time PCR thermocycler is not capable of measuring the dye Dyomics 681. If you do not have the necessary real-time PCR equipment, let Microsynth run your assay(s) of choice on a fee-for-service basis. In this context see also the submenu "Real-Time PCR – Project-Based Analysis Services".


References and Additional Technical Information

AllAll A

[1] Köppel R. et al. Two tetraplex real-time PCR for the detection and quantification of DNA from eight allergens in food. Eur. Food. Res. Technol. 230, 367-374 (2009).

 

AllAll B

[2] Köppel R. et al. Two tetraplex real-time PCR for the detection and quantification of DNA from eight allergens in food. Eur. Food. Res. Technol. 230, 367-374 (2009).

 

AllMeat

[4] Eugster A. et al. Quantification of beef, pork, chicken and turkey proportions in sausages: use of matrix-adapted standards and comparison of single versus multiplex PCR in an interlaboratory trial. Eur. Food. Res. Technol. 230, 55-61 (2009).

[5] Köppel R. et al. Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, chicken and turkey. Eur. Food. Res. Technol. 227,1199-1203 (2008).

[6] Accessories: Calculation sheet for normalisation to 100%

 

AllHorse

[7] Proficiency test done, publication submitted.

[8] Accessories: Calculation sheet for normalisation to 100%

[17] Köppel R. et al. Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, horse and sheep. Eur. Food. Res. Technol. 232,151-155 (2011).

 

AllMilk

[9]  Rentsch R. et al. Interlaboratory validation of two multiplex quantitative real-time PCR methods to determine species DNA of cow, sheep and goat as a measure of milk proportions in cheese European Eur. Food. Res. Technol. 236, 217-227 (2013).


AllGVOScB

[10] Publication in preparation.


AllGVOScC

[11] Publication in preparation.

 

AllSoyA

[12] Köppel R. et al. Multiplex real-time PCR for the detection and quantification of DNA from four transgenic soy Mon89788, A5547-127, Roundup Ready, A2704-12 and lectin. Eur. Food. Res. Technol. 235, 23-28 (2012).

[13] Accessories: Calculation sheet for ΔΔct calculations

 

AllBakA IPC

[14] Köppel et al (2013) Nonaplex real-time PCR detection of Listeria monocytogenes, Campylobacter, Salmonella and enteropathogene E. coli after universal enrichement in food samples; European Food Research and Technology 237:315–322.

 

AllCamp

[15] Mayr, A.M. et al. Rapid detection and differentiation of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in food, using Multiplex Real-Time PCR. Journal of food protection. 73,241-250 (2010).


Optimal Taq-polymerases for multiplex application like AllAllA,B, AllMeat, AllHorse, AllSoy and AllGVOScC

[16] Köppel R. et al. Proposal for a performance factor to characterize real-time PCR systems, evaluate premixed DNA-Polymerases and assign minimal performance criteria for individual quantitative PCR-runs. In press.

 

AllColi
[18] Pavliovic et al. Development of a Multiplex Real-Time Polymerase Chain Reaction for simultaneous Detection of enterohemorragic Escherichia coli and enteropathogenic Escherichia coli Strains. Foodborn Pathogens and Disease. 7,801-808 (2010).

 

AllPaté

[19] Köppel R. et al. Multiplex real-time PCR for the detection and quantification of DNA from duck, goose, chicken, turkey and pork; European Food Research and Technology Volume 236: 1093-1098 (2013).

 

AssignAnimal

[20] N.V. Morf et al (2013) A multiplex PCR method to identify bushmeat species in wildlife forensics Forensic Science International: Genetics Supplement Series, doi.org/10.1016/j.fsigss.2013.10.104.

 

 

 



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