DNA/RNA Isolation

In the modern world of DNA and RNA analysis, e.g. in Sanger sequencing, genotyping, real-time PCR or next-generation sequencing, it is generally known that the quality and purity of the isolated DNA or RNA has a significant impact on the final outcome.

 

Therefore, it is of paramount importance to rely on an optimized and well-proven isolation system when starting an experiment or project with a specific DNA/RNA source.

 

Microsynth looks back on more than 11 years of experience in DNA/RNA isolation from different and difficult matrices like plant material, food or stool. Our know-how in this area may help you to improve your overall productivity by allowing you to spend more time evolving your experiments and analyzing your data rather than expending time and effort on optimizing your DNA/RNA isolation approach.

A well-proven process for nucleic acid isolation ensures that the following 3 steps are professionally handled:

1. Effective disruption of the cellular structure to generate a lysate
2. Clean separation of the soluble nucleic acids from cell debris and other insoluble materials
3. Separation of nucleic acids from soluble proteins
4. Final high-level purification of nucleic acids

The following table gives you an overview about the various matrices where we have solid experience in isolating DNA, RNA or plasmid DNA. In this context it is important to note that we also have vast experience in the area of laboratory automation. Therefore, we are able to develop isolation protocols for our customers which also work under high-throughput conditions.

DNA/RNA Source	DNA/RNA Isolation Service
	DNA	RNA	Plasmid
Animal Tissues & Cells	yes	yes	n.a.
Biofilm	yes	yes	n.a.
Blood	yes	yes	n.a.
Fecal	yes	yes	n.a.
Food	yes	yes	n.a.
Microbes	yes	yes	yes
Plant	yes	yes	n.a.
Soil	yes	yes	n.a.
Water	yes	yes	n.a.