Modified DNA Oligonucleotides — Best Quality

According to your demand or specific application we can produce modified oligonucleotides meeting the highest requirements. On a routine basis we offer fluorescent 5'-end-labeled oligonucleotides at 3 slightly different purity levels:

• Purity Level 1: Proprietary PAGE
• Purity Level 2: Proprietary PAGE & NAP
• Purity Level 3: Proprietary PAGE & NAP Combined with Aseptic Handling

To learn more about product characteristics and indication of price ranges, please see the tables below. To obtain price details, please login to our webshop and download the current price list.

 

Additional Service Feature

Purification Service: Microsynth’s proprietary PAGE purification system and protocol has an excellent reputation in its field. For instance, many customers send us their VIC^TM, NED^TM, PET^TM labeled oligonucleotides for additional purification. Please note that due to patent issues, Microsynth may not be allowed to tag your desired premium oligo with any dye available on the market. However, in most cases we are able to suggest an equivalent alternative dye to fit your needs. Therefore, please approach us when you are looking for a cost-effective generic dye or when you are just looking for the best purification procedure available on the market.

How to Order?

• Important: Please first get in contact with Microsynth and request an offer!
• Enter our webshop
• Click on DNA and metRNA in the "Oligonucleotide" domain
• Select Upload Entry by using our convenient Excel Template (can be requested at administration@microsynth.ch)

Purity level 1: Proprietary PAGE

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All oligos produced are subjected to a proprietary PAGE system and protocol to yield products that are, on average, >98% pure.

Product Characteristics:

Type of 5’ Label	Guranteed Quantity Delivered [nmol]			Special Aseptic Handling during Production	Purification		Documen-tation1
	20	100	400		PAGE	NAP
FAM	x	x	x		x		ExCoA
JOE / Yakima Yellow	x	x	x		x		ExCoA
TAMRA	x	x	x		x		ExCoA
ROX	x	x	x		x		ExCoA
ATTO 425 / 532 / 550 / 565 / 620 / 647N	x	x	x		x		ExCoA
Dyomics 510XL / 530 / 630 /681/ 781	x	x	x		x		ExCoA

 

Potential Applications:

• Gene expression profiling
• MLPA (Multiplex ligation-dependent probe amplification
• TRFLP (Terminal restriction fragment length polymorphism)

Purity Level 2: Proprietary PAGE & NAP

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All oligos produced are subjected to a proprietary PAGE system and protocol to yield products that are, on average, >98% pure. A final desalting step by means of NAP treatment supports the further removal of disruptive dye blobs.

Product Characteristics:

Type of 5’ Label	Guranteed Quantity Delivered [nmol]			Special Aseptic Handling during Production	Purification		Documen-tation1
	20	100	400		PAGE	NAP
FAM	x	x	x		x	x	ExCoA
JOE /Yakima Yellow	x	x	x		x	x	ExCoA
TAMRA	x	x	x		x	x	ExCoA
ROX	x	x	x		x	x	ExCoA
ATTO 425 / 532 / 550 / 565 / 620 / 647N	x	x	x		x	x	ExCoA
Dyomics 510XL / 530 / 630 /681/ 781	x	x	x		x	x	ExCoA

 

Potential Applications:

• Within diagnostics kits to be used for fragment analysis of plant or animals
• Microsatellite analysis
• AFLP analysis (amplified fragment length polymorphisms)
• Mutation detection

Purity Level 3: Proprietary PAGE & NAP Combined with Aseptic Handling

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All oligos produced are subjected to a proprietary PAGE system and protocol to yield products that are, on average, >98% pure. A final desalting step by means of NAP treatment supports the further removal of disruptive dye blobs. In order to minimize contamination of the synthesized oligos with extrinsic DNA traces, Microsynth has implemented following set of proven measures:

• UV irradiation of all disposable materials in direct contact with the oligonucleotide to be synthesized
• Special cleaning procedure for production devices and dedicated production areas
• Dedicated SOPs

Product Characteristics:

Type of 5’ Label	Guranteed Quantity Delivered [nmol]			Special Aseptic Handling during Production	Purification		Documen-tation1
	20	100	400		PAGE	NAP
FAM	x	x	x	x	x	x	ExCoA
JOE/Yakima Yellow	x	x	x	x	x	x	ExCoA
TAMRA	x	x	x	x	x	x	ExCoA
ROX	x	x	x	x	x	x	ExCoA
ATTO 425 / 532 / 550 / 565 / 620 / 647N	x	x	x	x	x	x	ExCoA
Dyomics 510XL / 530 / 630 /681/ 781	x	x	x	x	x	x	ExCoA

    

Potential Applications:

• Within diagnostics kits to be used for human fragment analysis (e.g. forensic applications)
• Microsatellite analysis (human samples)
• AFLP analysis (amplified fragment length polymorphisms) (human samples)
• Mutation detection (human samples)
• MSI analysis (microsatellite instability analysis) (human samples)
• STR (human samples)

 

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¹ will be provided on request