Antisense Oligos


Oligonucleotides to be used in antisense experiments must be modified to increase their resistance against nucleases. There are several possibilities to stabilize oligos and to avoid enzymatic degradation during in vitro or in vivo applications. Microsynth can offer you the following two modifications:
  • PTO (phosphorothioates) modifications: PTOs contain one sulfur atom in place of an oxygen atom in the internucleotide linkage of DNA or RNA. This modification of the normal phosphodiester backbone is characterized by an increased cell uptake, high nuclease resistance and elicitation of RNAse H activity.
  • 2'-O-Me-RNA modifications: The incorporation of 2'-O-Me-RNA nucleotides induces a resistance to a wide variety of ribo- and deoxyribonucleases. This enables the formation of more stable hybrids with complementary RNA strands than would be the case for non-modified DNA and RNA sequences.
To learn more about specifications of these two types of modifications, please see the table below. To obtain information about our prices, please login to our webshop  and download the current price list.

Key Specifications:

Modifications

Position

Synthesis scale [µmol]1

Purification2

5’

3’

Int.

0.04

0.2

1.0

15

HPLC + Dialysis

PTO

 

 

x

x

x

x

x

x

2’-O-Me-RNA

x

x

x

 

x

x

x

x

 

How to Order?

  • Enter our webshop
  • Click on DNA and metRNA in the "Oligonucleotide" domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel template (can be downloaded from our webshop).
  • If you want to order PTO modified oligos, please mark PTO bonds by inserting an asterisk between the bases, e.g. AAA*T*G*CCC*AAA.
  • If you want to incorporate 2’-O-Me-RNA bases within your DNA sequences3, please proceed as follows:
    • Replace special bases inside the sequence by the figures "5,6,7,8" (e.g. 2’-O-Me-RNA-A via "5", 2’-O-Me-RNA-C via "6" etc.)
    • Specify the type of special bases under "Inner Modification" (e.g. by selecting 2’-O-Me-RNA-A from the drop down menu under "Inner Modification (5=...)" etc.)


1 The synthesis scale represents the initial amount of 3' bases (starting material). For antisense oligos apply the same guaranteed yields as for unmodified oligos (see also under DNA Oligos in the Standard Price Segment).
2 We strongly recommend to purify antisense oligos via HPLC followed by dialysis. This puts your oligos into a physiological state which is optimal for use in cells or live animals (toxicological NH4+ ions are replaced by Na+ ions).

3
If you want to order oligonucleotides consisting entirely of 2’-O-Me-RNA bases, please first click on DNA and metRNA in the "Oligonucleotide" domain when entering the webshop. Then click on Normal Entry and
select metRNA in the field "Type".















Questions?
Write an e-mail or call us at +41-71-722 83 33

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