DNA Oligos — Antisense

Oligonucleotides to be used in antisense experiments must be modified to increase their resistance against nucleases. There are several possibilities to stabilize oligos and to avoid enzymatic degradation during in vitro or in vivo applications. Microsynth can offer you the following two modifications:
  • PTO (phosphorothioates) modifications: PTOs contain one sulfur atom in place of an oxygen atom in the internucleotide linkage of DNA or RNA. This modification of the normal phosphodiester backbone is characterized by an increased cell uptake, high nuclease resistance and elicitation of RNAse H activity.
  • 2'-O-Me-RNA modifications: The incorporation of 2'-O-Me-RNA nucleotides induces a resistance to a wide variety of ribo- and deoxyribonucleases. This enables the formation of more stable hybrids with complementary RNA strands than would be the case for non-modified DNA and RNA sequences.
To learn more about specifications of these two types of modifications, please see the table below. To obtain information about our prices, please login to our webshop  and download the current price list.

Key Specifications:

Modifications

Position

Synthesis scale [µmol]1

Purification2

5’

3’

Int.

0.04

0.2

1.0

15

HPLC + Dialysis

PTO

 

 

x

x

x

x

x

x

2’-O-Me-RNA

x

x

x

 

x

x

x

x

 

How to Order?

  • Enter our webshop
  • Click on DNA and metRNA in the "Oligonucleotide" domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel template (can be downloaded from our webshop).
  • If you want to order PTO modified oligos, please mark PTO bonds by inserting an asterisk between the bases, e.g. AAA*T*G*CCC*AAA. If you want to incorporate 2’-O-Me-RNA bases within your DNA sequences3, please first specify each DNA base to be replaced by 2’-O-Me-RNA with “X”. In a second step you have to specify the type of 2’-O-Me-RNA base. X can be A, U, C or G. In the "Normal Entry" mode this can be done by replying to the confirmation e-mail whereas in the "Upload Entry" mode this will be done by using the field “special comments” in the Excel file.

 


1 We strongly recommend selecting HPLC followed by dialysis as purification in order to achieve physiological conditions.
2 The same guaranteed yields apply as for unmodified oligos (see also under DNA Oligos in the Standard Price Segment)
.
3 If you want to order oligonucleotides consisting entirely of 2’-O-Me-RNA bases, please first click on DNA and metRNA in the "Oligonucleotide" domain when entering the webshop. Then click on Normal Entry and select metRNA in the field "Type".















Questions?
Write an e-mail or call us at +41-71-722 83 33

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