Antisense Oligos

Oligonucleotides to be used in antisense experiments must be modified to increase their resistance against nucleases. There are several possibilities to protect oligonucleotides from enzymatic degradation during in vitro or in vivo applications. Microsynth can offer you the following three modifications::
  • PTO (phosphorothioates) modifications: PTOs contain one sulfur atom in place of an oxygen atom in the internucleotide linkage of DNA or RNA. This modification of the normal phosphodiester backbone is characterized by an increased cell uptake, high nuclease resistance and elicitation of RNAse H activity.
  • 2'-O-Me-RNA modifications: The incorporation of 2'-O-Methyl RNA nucleotides induces a resistance to a wide variety of nucleases, in particular RNase. Furthermore, 2’-OMe oligonucleotides show slightly increased affinity towards their complementary mRNA target sequence, thereby forming more stable hybrid duplexes compared to their non-modified DNA or RNA counterparts. This enables the formation of more stable hybrids with complementary RNA strands than would be the case for non-modified DNA and RNA sequences.
  • 2'-MOE-RNA modifications: Oligonucleotides incorporating 2'-O-methoxyethyl (MOE)-modified nucleotides, can support most, if not all antisense mechanisms of action. Further key distinctive characteristics are nuclease resistance, lower toxicity, superior target binding specificity, as well as increased affinity towards complementary RNA. For more detailed information about 2'-MOE antisense oligonucleotides from Microsynth, please see the flyer under "Related Downloads".
To learn more about specifications of these three types of modifications, please see the table below. To obtain information about our prices, please login to our webshop and download the current price list.

Key Specifications:

Modifications

Position

Synthesis scale [µmol]

Purification1

5’

3’

Int.

0.04

0.2

1.0

15

LS*

HPLC + Dialysis

PTO

 

 

x

x

x

x

x

x

x

2’-O-Me-RNA2

x

x

x

 

x

x

x

x

x

2'-MOE-RNA x x x   x x x x x
  * LS: abbreviation for large-scale synthesis with synthesis yields up to gram amounts

How to Order?

  • Enter our webshop
  • Click on DNA in the blue "DNA/RNA Synthesis" domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel template (can be downloaded from our webshop).
  • PTO modified oligonucleotides: please mark PTO bonds by inserting an asterisk between the bases, e.g. AAA*T*G*CCC*AAA.
  • 2’-O-Me-RNA (Selective incorporation of 2’-O-Me-RNA bases into DNA):
    • Enter 5,6,7,8 for each distinct base to be replaced inside the sequence
    • Define Inner Modification (5=…) by selecting the desired modification from the drop down menu (e.g. 5=…2’-O-Methyl-RNA-A, 6=…2’-O-Methyl-RNA-C etc.)
  • 2’-MOE-RNA (Selective incorporation of 2’-MOE-RNA bases into DNA):
    • Enter 5,6,7,8 for each distinct base to be replaced inside the sequence
    • Define Inner Modification (5=…) by selecting the desired modification from the drop down menu (e.g. 5=…2’-Methoxyethyl-ribo-A, 6=…2’-Methoxyethyl-ribo-mC etc.)

1 We strongly recommend selecting HPLC followed by dialysis as purification in order to achieve physiological conditions. The same guaranteed yields apply as for unmodified oligos (see also under DNA Oligos in the Standard Price Segment).
2 If you want to order oligonucleotides consisting entirely of 2’-O-Me-RNA bases, please first click on 2'-O-Methyl RNA in the blue "DNA/RNA Synthesis" domain.






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