DNA Oligos — Dual-Labeled Probes Overview
Microsynth's offers a wide selection of fluorophores and quenchers which will support most real-time quantitative PCR instruments and multiplex analysis systems. Here are some arguments to use dual-labeled probes, especially those from Microsynth.
Main Product Features and Benefits:
1 Nucleic acids with enhanced hybridization properties (e.g. C5-propynes, MGBs, LNAs) are usually used to:
Main Product Features and Benefits:
- Broad portfolio of probes: availability of various fluorophore and quencher combinations (see also the section “Fluorophores and Quenchers” under Related Links)
- High quality: all probes are MALDI-TOF controlled before shipment
- Minimum delivery guarantee (delivered amount is on average at least 3 times higher)
- Fast delivery (within 3 working days on average)
- Competitive and transparent pricing: prices for dual-labeled probes include all DNA bases, fluorophores & quenchers as well as a thorough purification procedure (HPLC or even PAGE)
- Free of charge: free probe design service available; design of dual-labeled probes and primer pairs on request
- Additional services:
- Design, synthesis and functionality testing of primer-probe sets for real-time PCR (see also the flyer "Customized qPCR Assays" under Related Downloads)
- C-5 propynyl modification of your probe (see also the flyer "MGB and LNA Substitutes" under Related Downloads)¹
- Aliquotation of probes and shipment within 5 tubes on request2
- Gene expression
- Multiplex assay development
- Pathogen detection
- Viral load quantitation
- Microarray validation
- si/shRNA knockdown
- Gene copy determination
- Mutation detection
- Allelic discrimination
- SNP detection
- Enter our webshop
- Click on DNA and metRNA in the "Oligonucleotide" domain
- Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel Template (can be downloaded during ordering)
- Simply select your desired 5' and 3' modification, in this way your oligo is recognized and processed as a TaqMan probe (including thorough HPLC or PAGE purification)
1 Nucleic acids with enhanced hybridization properties (e.g. C5-propynes, MGBs, LNAs) are usually used to:
- To increase the thermal stability and hybridization specificity of your probe
- To get greater accuracy in gene quantitation and allelic discrimination
- To facilitate the probe design of problematic target sequences
