DNA Oligos — Dual-Labeled Probes Overview

Whether your application is genotyping, gene expression, pathogen detection, or another kind of application, Microsynth's wide selection of fluorophores and quenchers will support most real-time quantitative PCR instruments and multiplex analysis systems. 

Benefits of using dual-labeled probes include:
  • Design simplicity for sequence specificity
  • Increased sensitivity
  • Extensive availability of fluorophore combinations
Use alternative modifications to MGB probes and LNA chemistry to:
  • Increase the thermal stability and hybridization specificity of your probe
  • To get greater accuracy in gene quantitation and allelic discrimination
  • To facilitate the probe design of problematic target sequences
Main Product Features and Benefits:
  • Competitive and transparent pricing: prices for dual-labeled probes include all DNA bases, fluorophores & quenchers as well as a two-step purification procedure (HPLC/PAGE)
  • High quality: all probes are MALDI-TOF controlled before shipment
  • High quantity delivered (≥1.5 OD260, current delivered amount on average is 2-3 times higher)
  • Enhanced stability: aliquotation of probes and shipment within 5 tubes¹
  • Fast delivery (within 3 working days on average)
  • Free of charge: free probe design service available; design of dual-labeled probes and primer pairs on request
  • Additional services:
    • Design, synthesis and functionality testing of primer-probe sets for real-time PCR (see also the flyer "Primer Probes Sets for Real-time PCR" under Related Downloads)
    • C-5 propynyl modification of your probe (see also the flyer "MGB and LNA Substitutes" under Related Downloads)


1 If you wish only one tube please proceed as follows during the ordering process: Either immediately return the order confirmation e-mail and note “only one tube”; or alternatively write “only one tube” into the field “special comments” when using the upload entry mode

  

 

 

 

 

 

 






Questions?
Write an e-mail or call us at +41-71-722 83 33


Related Downloads
MGB and LNA Substitutes.pdf
Informs how you can replace MGB and LNA-modified probes

Primer-Probe Sets for Real-Time PCR.pdf
Informs how Microsynth designs, synthesizes and tests the functionality of primer-probe sets for real-time PCR

Notification C-5 Propynes.pdf
Notification about the usage of C-5 Propynes for research only.


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