Modified DNA Oligos

Microsynth offers a wide variety of modifications (5’, 3’ and internal) for DNA oligonucleotides. To give you a better overview, they have been categorized as
To learn more about the key specifications of a modification, please see the tables below. To obtain information about our prices, please login to our webshop and download the current price list.

Important - Please Note!
Microsynth is continuously expanding its portfolio of dyes which can be used as cost-effective alternatives to patent-protected dyes. Examples are the following: Yakima Yellow or ATTO 532 for VIC™, ATTO550 for NED™, ATTO565 for PET™, Dyomics630 for LIZ™.  LI-COR Sequencer compatible dyes Dy681/781 as alternatives for IRDyes® 700/800 are available. If your desired modification is not listed below, please contact us for availability.

For modified DNA oligonucleotides within our Standard Segment, we do not provide minimal or guaranteed delivery amounts. In this context, please see also under DNA Oligos – Best Quality within our Premium Segment.

How to Order?
  • Enter our webshop
  • Click on DNA and metRNA in the "Oligonucleotide" domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel Template (can be downloaded during ordering)
  • Follow the further instructions

 
DNA Base and/or Sugar-phosphate Backbone Modifications
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Type of Modification

Position

Synthesis scale [µmol]

Purification

5’

3’

Int.

0.04

0.2

1.0

Des

HPLC

PAGE

Amino-dT     x   x x x x x
Azide-dT     x   x x
x x
2-Aminopurine x   x   x x x x x
5-Br-dC and 5-Br-dU*
    x   x x   x x

2'-Deoxyinosine (INO)

 

x

x

 

x

x

 

x

x

3'-Deoxy-A, C, G and T1
  x     x x   x x

5-Met-dC

 

x

x

 

x

x

x

 

 

5-OH-Met-dC*

 

 

x

 

x

x

 

x

x

N6-Met-dA*

x
  x x x x x
Mixed Bases
 x x
x  x x x   x x
Phosphoreothioate bases (PTO)

   x  x x x x x
x
Spacer 18 (HEG)
 
 x   x x x x x

Spacer C3

 

x

x

 

x

x

x

x

x

Spacer C12

 

 

x

 

x

x

x

x

x

dSpacer (abasic site)

 

 

x

 

x

x

 

x

x

TIPS-Alkyne-(C8)-dT*

 

 

x

 

x

x

 

x

x

TMS-Alkyne-(C8)-dT*     x            
* on request (as well as other modifications not listed in above table)     

 

 

 Non Fluorescent Modifications >>to the top
   

Type of Modification

Position

Synthesis scale [µmol]*

Purification

5’

3’

Int.

0.04

0.2

1.0

Des

HPLC

PAGE

Alkyne (Click Chemistry)

 

x

 

 

x

x

x

x

x

Aminolink (C6) x     x x x x x x

Aminolink (C6 or C7)2

 

x

 

 

x

x

x

x

x

Azide (Click Chemistry) x       x x x   x
  x     x x x x x
Azide dT (Click Chemistry)     x   x x   x x

Biotin (C6)

x

 

 

x

x

x

 

x

x

Biotin (TEG)   x     x x   x x
Biotin dT     x   x x x x x

Cholesterol

 

x

 

 

x

x

x

 

 

Digoxigenin

x

x

 

 

x

x

 

x

x

DBCO (Cu-free Click Chem.) x

  x x x x x
DBCO dT (Cu-free Click Chem.)     x   x x   x x
Hexynyl (Click Chemistry) x       x x x x
x
Inverted Thymidine   x     x x x x x

Phosphorylation

x

 

 

x

x

x

x

x

x

 

x

 

 

x

x

x

x

x

Thiol (C6)

x

 

 

 

x

x

 

x

x

Thiol (C3)

 

x

 

 

x

x

 

x

x

Other modifications on request      

* 15 µmol scale available on request

 

 


 Fluorescent or Dye Modifications >>to the top

Type of Modification

Position

Synthesis Scale [µmol]*

Purification

5’

3’

Int.

0.04

0.2

1.0

Des

HPLC

PAGE

AlexaFluor 350/430

x

x

 

 

x

x

 

x

x

AlexaFluor 430 dT     x   x x   x x

ATTO 425/532/550/590/565/620/647N

x

 

 

x

x

x

 

x

x

 

x

 

 

x

x

 

x

x

ATTO 550/647N dT

 

 

x

 

x

x

 

x

x

BHQ-1/BHQ-2   x     x x   x x

Cy3/5/5.5/Cy7

x

 

 

x

x

x

 

x

x

 

x

 

 

x

x

 

x

x

Dabcyl

 

x

 

 

x

x

 

x

x

Dyomics 510XL/530/630/681

x

 

 

x

x

x

 

x

x

 

x

 

 

x

x

 

x

x

Dyomics 510XL/530 dT     x   x x   x x

Dyomics 781

x

 

 

x

x

x

 

 

x

 

x

 

 

x

x

 

 

x

FAM

x

 

 

x

x

x

 

x

x

 

x

 

 

x

x

 

 

x

FAM dT

 

 

x

 

x

x

 

x

x

HEX x     x x x   x x

Joe

x

x

 

 

x

x

 

x

x

Joe dT     x   x x   x x

Rox

x

x

 

 

x

x

 

x

x

Rox dT     x   x x   x x

Tamra

x

x

 

 

x

x

 

x

x

Tamra dT     x   x x   x x

TET

x

 

 

x

x

x

 

x

x

Texas Red x       x x   x x

Yakima Yellow

x

 

 

 

x

x

 

x

x

Other modifications on request

* 15 µmol scale available on request

 

 


1  3'-Deoxy-A, C, G and T is functional equivalent to 2'3' Dideoxy A/G/C/T

2  3' Aminolink C7 is used for oligos >50 bases


 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 






Questions?
Write an e-mail or call us at +41-71-722 83 33


Related Downloads
Dye Alternatives.pdf
Informs about cost-effective dye alternatives for qPCR or genotyping experiments


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