DNA Oligos — Modified

Microsynth offers a wide variety of modifications (5’, 3’ and internal) for DNA oligonucleotides. To give you a better overview, they have been categorized as

• Modifications involving the DNA Base and/or the Sugar-phosphate Backbone
• Non Fluorescent Modifications
• Fluorescent or Dye Modifications
  

To learn more about the key specifications of a modification, please see the tables below. To obtain information about our prices, please login to our webshop and download the current price list.

Important - Please Note!
Microsynth is continuously expanding its portfolio of dyes which can be used as cost-effective alternatives to patent-protected dyes. Examples are the following: Yakima Yellow or ATTO 532 for VIC™, ATTO550 for NED™, ATTO565 for PET™, Dyomics630 for LIZ™.  LI-COR Sequencer compatible dyes Dy681/781 as alternatives for IRDyes^® 700/800 are available on request. If your desired modification is not listed below, please contact us for availability.

For modified DNA oligonucleotides within our Standard Segment, we do not provide minimal or guaranteed delivery amounts. In this context, please see also under DNA Oligos – Best Quality within our Premium Segment.

How to Order?

• Enter our webshop
• Click on DNA and metRNA in the "Oligonucleotide" domain
• Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel Template (can be downloaded during ordering)
• Follow the further instructions

 

DNA Base and/or Sugar-phosphate Backbone Modifications

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Type of Modification	Position			Synthesis scale [µmol]			Purification
	5’	3’	Int.	0.04	0.2	1.0	Des	HPLC	PAGE
5-Br-dC and 5-Br-dU			x		x	x	x	x	x
2-Aminopurine	x		x		x	x	x	x	x
2’-Deoxyinosine (INO)		x	x		x	x	x	x	x
2’-Deoxyuridine (dU)		x	x		x	x	x	x	x
3’-Deoxy-dA, dC, dG and dT1		x			x	x	x	x	x
5-Met-dC			x		x	x	x	x	x
N6-Met-dA			x		x	x	x	x	x
Mixed Bases	x	x	x	x	x	x	x	x	x
Phosphorothioate bases (PTO)			x	x	x	x	x	x	x
Spacer C18 (HEG)			x		x	x	x	x	x
Spacer C3		x	x		x	x	x	x	x
Spacer C12			x		x	x	x	x	x
dSpacer (abasic site)			x		x	x	x	x	x
Other modifications (e.g. 5-Br-dC, 5-Br-dU, N6-Met-dA, 5-OH-Met-dC) on request

 

 

Non Fluorescent Modifications

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Type of Modification	Position			Synthesis scale [µmol]*			Purification
	5’	3’	Int.	0.04	0.2	1.0	Des	HPLC	PAGE
Aminolink (C6)	x			x	x	x	x	x	x
Aminolink (C7)		x			x	x	x	x	x
Biotin (C6)	x			x	x	x		x	x
Biotin (TEG)		x			x	x		x	x
Cholesterol		x			x	x	x
Digoxigenin	x	x			x	x		x	x
Hexynyl (Click Chemistry)	x				x	x	x	x	x
Phosphorylation	x			x	x	x	x	x	x
		x			x	x	x	x	x
Thiol (C6)	x				x	x		x	x
Thiol (C3)		x			x	x		x	x
Other modifications on request
* 15 µmol scale available on request

 

 

Fluorescent or Dye Modifications

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Type of Modification	Position			Synthesis Scale [µmol]*			Purification
	5’	3’	Int.	0.04	0.02	1.0	Des	HPLC	PAGE
AlexaFluor 350 / 430	x	x			x	x		x	x
ATTO 425 / 532 / 550 / 565 / 620 / 647N	x			x	x	x		x	x
		x			x	x		x	x
BHQ-1 / BHQ-2		x			x	x		x	x
Cy3 / 5 / 5.5	x	--			x	x		x	x
Cy7	x	x			x	x		x	x
Dabcyl		x			x	x		x	x
Dyomics 510XL / 530 / 630 / 681	x			x	x	x		x	x
		x			x	x		x	x
Dyomics 781	x			x	x	x			x
		x			x	x			x
FAM	x			x	x	x		x	x
		x			x	x			x
HEX	x			x	x	x		x	x
Joe	x	x			x	x		x	x
QSY-7 / QSY-21	x	x			x	x		x	x
Rox	x	x			x	x		x	x
Tamra	x	x			x	x		x	x
TET	x			x	x	x		x	x
Yakima Yellow	x				x	x		x	x
Other modifications on request
* 15 µmol scale available on request

 

 

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¹  3'-Deoxy-dA, dC, dG and dT is equivalent to 2'3' Dideoxy A/G/CT