DNA Oligos

We use state-of-the art synthesizer technologies and purification methods to offer you unmodified DNA oligos with 4 different purity levels:
To learn more about product characteristics, please see the tables below. To obtain information about our prices, please login to our webshop and download the current price list.

How to Order? 
  • Enter our webshop
  • Click on DNA and metRNA in the Oligonucleotide domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel Template (can be downloaded during ordering )
  • Follow the further instructions
 
 Desalted Oligos >>to the top

Product Characteristics:

Synthesis scale1

Length Restriction

Guaranteed Yield

Average Yield

Production Time [wd]

[OD260]2

[nmol]3

[OD260]2

[nmol]3

Genomics

13 - 39

2

10

8

40

1

0.04 µmol

13 - 80

3

15

9

45

1

0.2 µmol

6 - 1254

10

65

20

100

1

1.0 µmol

6 - 80

50

250

80

400

1

15 µmol

13 - 50

700

3‘500

1000

5000

2


Potential Applications:

  • PCR
  • DNA sequencing
  • Probing
  • Mobility shift or hybridization
 
 HPLC Purified Oligos >>to the top

Product Characteristics:

Synthesis scale1

Length Restriction

Guaranteed Yield

Average Yield

Production Time [wd]

[OD260]2

[nmol]3

[OD260]2

[nmol]3

Genomics

not available

0.04 µmol

<51

1

5

4

20

2

0.2 µmol

3

15

7

35

2

1.0 µmol

15

75

26

130

2

15 µmol

300

1'500

500

2'500

3


Potential Applications:
  • Molecular cloning
  • DNA fingerprinting
  • Real-Time PCR
  • FISH

 HPLC Purified & Dialysed Oligos >>to the top

Product Characteristics:

Synthesis scale1

Length Restriction

Guaranteed Yield

Average Yield

Production Time [wd]

[OD260]2

[nmol]3

[OD260]2

[nmol]3

Genomics

not available

0.04 µmol

not available

0.2 µmol

<51

3

15

6

30

3

1.0 µmol

15

75

20

100

3

15 µmol

200

1‘000

400

2'000

4


Potential Applications:
  • Antisense experiments
  • Cell culture studies
  • Analytics (NMR, MS etc.)
 
 PAGE Purified Oligos >>to the top

Product Characteristics:

Synthesis scale1

Length Restriction

Guaranteed Yield

Average Yield

Production Time [wd]

[OD260]2

[nmol]3

[OD260]2

[nmol]3

Genomics

not available

0.04 µmol

13 - 80

0.5

2.5

6

30

2

0.2 µmol

8 - 1254

1

5

8

40

2

1.0 µmol

8 - 80

7

35

20

100

2

15 µmol

not available


Potential Applications:

  • Molecular cloning
  • Mutagenesis
  • DNA fingerprinting
  • In situ hybridization
  • Gene synthesis


¹ The synthesis scale represents the initial amount of 3' bases (starting material).
² Guaranteed and average yields in OD are valid for unmodified oligos >20mer only.
3 Yields indicated in nmol represent an example calculation for a 20mer. For this calculation the following rule of thumb equation was applied: nmol of oligo = OD x 100/length of oligo. Please note that this calculation is based on sequences with virtually homogenous distribution of the 4 DNA bases; it may vary for sequences with high GC contents >70% etc.

4 Oligos longer than 125 DNA bases on request (we would like to discuss the proposed experiment/application with you beforehand in order to guarantee the best possible outcome)




















































Questions?
Write an e-mail or call us at +41-71-722 83 33


Related Downloads
TSG Mutations in Oligonucleotides.pdf
Troubleshooting guide with especial focus on the use of oligonucleotides during molecular cloning projects


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