Antisense Oligos with Phosphorothioate (PTO) / 2'-O-methyl-RNA- modification
- Oligos that are used in antisense experiments must be modified to achieve a higher nuclease resistance. There are several types of such modifications. Microsynth offers two possibilities:
- Modification of the normal phosphodiester backbone (phosphorothioates) which is characterized by an increased cell uptake, high nuclease resistance and elicitation of RNAse H activity. We can produce phosphorothioate linkages at any position of your choice.
- The incorporation of 2'-OMe-nucleotides (2'-OMe-RNA) which induces a resistance to a wide variety of ribo- and deoxyribonucleases. This enables the formation of more stable hybrids with complementary RNA strands than equivalent non-modified DNA and RNA sequences.
- If you wish to use antisense Oligos in cell culture, it is important to remove all of the salts within the Oligo sample. We recommend ordering of NMR grade purified Oligos. This grade of purification is carried out by reverse phase chromatography and subsequent dialysis.
- 0.04 µmol (only PTO-modification), 0.2 µmol, 1.0 µmol and 15 µmol scales are available (same guaranteed yields as for unmodified Oligos apply).
Scale | Phosphorothioates (PTO) | 2'-O-methyl-RNA |
0.04 µmol | Product No. 0600 | not available |
0.2 µmol | Product No. 0600 | Product No. 0606 |
1.0 µmol | Product No. 0601 | Product No. 0607 |
15 µmol | Product No. 0602 | Product No. 0608 |
The same guaranteed yields apply as for unmodified Oligos.
