siRNA Stability Information
Our siRNAs are standardly delivered in solution as annealed duplexes. The in-solution delivery form may be a bit counter-intuitive, but as our tests confirm (see below) siRNAs are indeed very stable in solution.
Futhermore, delivering siRNAs in solution has major advantages for you:
- siRNAs are indeed ready-to-use. No dissolving and heating before use.
- siRNAs have enhanced activity due to loss of lyophilization-aggregates and problematic super-structures caused by the drying process.
- siRNAs in solution lead to less risks in contamination.
The siRNAs have an adjusted concentration of 40 uM and are in the following buffer (1x Annealing Buffer):
1x Annealing Buffer
10 mM Tris, pH 7.0
20 mM NaCl
We recommend you to dilute the siRNA to any desired concentration, aliquot it, and freeze it at -20 degrees until use. For prolonged storage, you may dry it down.
Stability Information:
In our test, several annealed siRNAs in solution were incubated at room temperature for several weeks and stability has been analyzed by MALDI-TOF MS-Analysis. The result is shown in the figure below:
T0d T30d
MALDI-TOF Analysis of siRNAs. On the left side, an siRNA was analysed at time point zero. Note the two strands of the siRNAs as two peaks in the spectrum (be aware that the peak size does not correspond to relative amounts!). The right figure shows a MALDI-TOF analysis of the same siRNAs after 30 days of incubation at room temperature. No degradation has occured - the siRNA is stable.
