Overview
TaqMan probes also called Double-Dye Oligonucleotides or dual-labelled probes are the most widely used type of probes. When low-copy-number detection and quantification are needed, sequence specific dual-labelled probes detect only one specific sequence in contrast to SYBR Green I. Using multiple different fluorescent dyes on the probe for different genes allows the development of multiplex detections. Microsynth offers a wide range of fluorophores and quenchers in various scales.
How to order TaqMan Probes
Please use the regular DNA oligonucleotide orderform. Simply choose your desired 5' and 3' modification, in this way your Oligo is recognized and processed as TaqMan probe (including two-step HPLC purification).
Important Information
- Prices for dual-labelled probes include base addition, dye labelling and two-step HPLC purification.
- All probes are MALDI-TOF MS-controlled before shipment.
- Dual-labelled probes are normally aliquoted in 5 tubes to achieve maximal stability and shipped lyophilized. If you wish only one tube please return the order confirmation e-mail with your special request immediately.
- Design of dual-labeled probes and primer pairs on request.
- Free probe design service available.
