RNA synthesis has become a standard as has DNA oligonucleotide synthesis. However, due to degradation-prone characteristics of RNA special precautions have to be taken. Additionally, the free 2'-OH needs to be protected during synthesis.

Microsynth uses state of the art TOM-chemistry for its RNA synthesis. The use of the 2'-O protective group has several advantages over traditional 2'-O protecting groups such as tBDMS and others. Advantages include the lack of 5'-2' chain elongations, better coupling efficiencies and more. This leads to measurable higher quality of our RNAs compared to our competitor's.

Our collaborators at the University of Zurich, Switzerland, compared our RNA with one of our leading competitor's. In their experiment, a 65-mer was in vitro translated to a peptide; its yield is directly dependent on the quality of the RNA at identical concentrations of RNA. The figures on the left demonstrate that Microsynth's RNA is superiour compared to our competitor's RNA.

Do you have any more questions concerning our synthesis chemistry? Please do not hesitate to contact us for more information.