Fire Blight PCR Detection Kit
This is based on a nested PCR reaction.
Main advantages are:
- Speed (the detection of fire blight is possible within 6 hours after the beginning)
- No precultivation of E. amylovora bacteria – direct PCR from stems, leaves, fruits or blossoms.
- The PCR reaction performs 67 cycles, this special high amplification exceeds by far the normal PCR procedures using just 35 cycles and results in a very high sensitivity, even with a very low starting amount of DNA. Therefore, this method is unaffected by the presence of PCR inhibitors.
- Sensitivity: 1 culturable E. amylovora cell is detectable with this PCR kit
- High standardization. Each production lot is checked at Microsynth and has to meet the quality standards mentioned on the supplied data sheet. Therefore, a lot-to-lot variation is excluded. The only source of variation is the way how the sample is taken from the plant.
Fire Blight Flyer: Download
QC System:
Each production lot is inspected by PCR with a positive and negative control. On the agarose gel, the positive control must show a clear band of twice the intensity compared to the internal control at 139bp. The negative control must show no fire blight band but the same intensity of the internal control.
Manual:
German version: Download
English version: Download
French version: Download
References:
Contact:
For further details and pricing information please contact Dr. Tobias Schmidheini at
t.schmidheini@microsynth.ch
Phone direct : +41 71 726 1209
