Editing of your Sequence
What is the difference between non-edited and edited sequencing reactions?
In automated sequencing, different fluorescent dyes absorb the excitation energy of a laser and allow DNA to be detected in the gel during electrophoresis. A software program then measures the signal strength of the fluorescent bands and creates a chromatogram. Thereafter, automated "base calling" is applied by the instrument which "translates" the chromatogram into a sequence.
Unfortunately, some artefacts cannot be recognized by a “base-caller” program, resulting in the translation of wrong bases or non-defined N-bases. Only experienced “eyes” can detect and correct such uncertainties.
more
