How Pure is Pure Enough?
In general, the purity level that you require depends on the effect that failed sequences (n-1, n-2,... Oligomers) will have on your experiments.
For some applications, desalted Oligos are satisfactory. For others, only PAGE purification can yield Oligos of sufficient purity. We recommend the use of purified Oligos for difficult projects.
Please note that yields vary according to the selected purification method.
Desalted Oligos
All Oligos are at least desalted to remove residual by-products from the synthesis, cleavage and deprotection procedures.
HPLC Purified Oligos
Oligos 50 bases or shorter can be HPLC purified using a Reverse Phase HPLC system. Residual, truncated DMT-off Oligo molecules from synthesis are therefore removed during the purification process, resulting in 90-95% purity.
PAGE Purified Oligos
Oligos 51 bases or longer should be PAGE purified. With excellent resolution resulting from PAGE, 96-99% purity can be achieved. In many cases, full length Oligos can be separated from Oligos which are just one base shorter. This purification grade is also recommended for applications including labelling of Oligos longer than 50mer.
