RNA Synthesis Scales
Synthesis scale* | Restrictions | Guaranteed yield for >20mer <40mer, unmodified, desalted |
0.04 µmol | 10-30mer | 4 OD260 |
0.2 µmol | 5-65mer** | 6 OD260 |
1 µmol | 5-65mer** | 16 OD260 |
15 µmol | 15-40mer | on request |
* The synthesis scale is the amount of 3' base that we start the synthesis with. It is NOT the expected final yield. The yield depends on the length of the Oligonucleotide, the coupling efficiency, and the base composition. The theoretical yield of the full length product can be determined using the formula 0.985n, wherein 0.985 represents the coupling efficiency and n the length of the Oligo. Hence, the theoretical yield of the full length product of a 50mer is 47% at a coupling rate of 0.985. Shorter Oligos can not be eliminated by desalting but instead by HPLC or PAGE. We recommend HPLC or PAGE purification for Oligos longer than 35-mer.
** Note that synthesis of longer oligos is possible, but yields are lower.
siRNA Synthesis Scales
Synthesis scale | Guaranteed yield (desalted, umodified)* |
0.04 µmol | 21 nmol |
0.2 µmol | 35 nmol |
1 µmol | 84 nmol |
15 µmol | on request |
* average yields are significantly higher
