DNA Synthesis Scales
Synthesis scale*) | Restrictions | Guaranteed yield for >20mer, unmodified, desalted | Average yield for >20mer, |
Genomics | 13-39mer, no modification, desalted only | 2 OD260 | 7 OD260 |
0.04 µmol | 13-70mer | 3 OD260 | 10 OD260 |
0.2 µmol | 6-125mer**) | 13 OD260 | 20 OD260 |
1.0 µmol | 6-80mer | 50 OD260 | 100 OD260 |
15 µmol | 6-50mer | 700 OD260 | 1000 OD260 |
*) The synthesis scale is the amount of 3'base that we start the synthesis with. It is NOT the expected final yield. The yield depends on the length of the Oligonucleotide, the coupling efficiency, and the base composition. The theoretical yield of the full length product can be determined using the formula 0.985n, wherein 0.985 represents the coupling efficiency and n the length of the Oligo. Hence, the theoretical yield of the full length product of a 50mer is 47% at a coupling rate of 0.985. Shorter Oligos can not be eliminated by desalting but instead by HPLC or PAGE. We recommend HPLC or PAGE purification for Oligos longer than 35-mer.
**) Longer oligos on request.
Important Information
- Please inquire for special offers if you plan an order with more than 50 Oligos. Request our convenient customized Excel sheet at administration(at)microsynth.ch
- When ordering mixed bases please use the IUPAC-code (M=A/C, R=A/G, W=A/T, Y=C/T, S=C/G, K=G/T, H=A/C/T, V=A/C/G, D=A/G/T, B=C/G/T, N=A/C/G/T). No additional charges will be applied for internal mixed base positions (wobbles).
