What is the best way to re-suspend Oligos?
- Do a short spin at max. speed in a centrifuge to collect the pellets at the bottom of the tubes.
- Add the appropriate amount of sterile water or buffer.
- Heat 5 mins. at 65 °C.
- Vortex or mix by pipetting vigorously up and down.
What is the best solvent for an Oligo?
For all Oligos (except those with fluorescent dyes) sterile water or buffer (e. g. 10 mM Tris-HCl pH 7.5) is fine. We recommend dissolving fluorescently labelled Oligos in 10 mM Tris pH 7.5 as degradation may occure at low pH (distilled water may have a pH of 6.0!). However Cy-labelled Oligos (Cy3, Cy3.5, Cy5, Cy5.5) degrade slowly at pH >7.5 and should therefore be dissolved at pH 6.0 -7.0.
Special Care for Dye-labelled Oligos
Some dyes (especially Cy-x dyes) are specially sensitive to light and should consequently be stored in light-tight containers. Furthermore, dyes may be sensitive to oxidation. Leave containers closed as long as possible and avoid repeated opening and closing and storage on the bench.
